《植物生理学报》 2015, 51(4): 509-516

耐旱相关基因SiUPF3的克隆表达及生物信息学分析

戴一明, 安利佳*, 李文利

大连理工大学生命科学与技术学院, 辽宁大连116024

通信作者：安利佳；E-mail: bioeng@dlut.edu.cn；Tel: 0411-84706365

摘　要：

Upf3蛋白参与一种保守的mRNA降解系统即无义介导衰变(nonsense-mediated decay, NMD)。它主要存在于真核细胞中参与降解异常的mRNA。本文通过比较‘晋谷34’正常生长和干旱胁迫下谷子表达谱基因相对表达量的变化幅度大小, 筛选得到一个响应干旱同时能够编码Upf3蛋白的基因, 经谷子基因组在线数据查询并比对得到全长基因Si009903m的编码序列(coding sequence, CDS), 克隆该基因的CDS并命名为SiUPF3。SiUPF3长1 515 bp, 编码504个氨基酸。经实时定量PCR分析表明SiUPF3受ABA途径调控, 并且在干旱胁迫下显著上调。

关键词：SiUPF3; 谷子; 耐旱基因; ABA途径; 实时定量PCR

收稿：2015-01-28   修定：2015-03-09

资助：辽宁省科技厅农业攻关项目(2011208001)。

Expression and Bioinformatics Analysis and Cloning of the Drought-Resistant Gene SiUPF3 in Setaria italica

DAI Yi-Ming, AN Li-Jia*, LI Wen-Li

School of Life Science & Biotechnology, Dalian University of Technology, Dalian, Liaoning 116024, China

Corresponding author: AN Li-Jia; E-mail: bioeng@dlut.edu.cn; Tel: 0411-84706365

Abstract:

Upf3 protein is one of the most important factors in the mRNA surveillance process. This process is a conservative mRNA degradation system which is mainly involved in the degradation of abnormal mRNA in eukaryotic cells. To study drought-tolerant genes, we compared the expression of ‘Jingu34’ under drought condition and normal one and filtrated drought-tolerant genes in the level of transcription. In this paper, we got the full length gene which is numbered Si009903m through searching the sequence within online Setaria italica genome data. Finally we cloned the coding sequence (CDS) of Si009903m and named it SiUPF3 for the Upf3 protein it encoded. The CDS of Si009903m is 1 515 bp and encodes 504 amino acids. Quantitative RT-PCR analysis infered that SiUPF3 is regulated by ABA signal network and significantly upregulated under drought.

Key words: SiUPF3; Setaria italica; drought-tolerant genes; ABA pathway; quantitative RT-PCR